>>>>The Global.asax be default has several methods (e.g. Application_Start, Session_Start, and so on). I need to add the ScriptManager call (as shown below) to the Global.asax
>>>>
>>>>

>>>>ScriptManager.ScriptResourceMapping.AddDefinition("jquery", new ScriptResourceDefinition {
>>>>
>>>>                Path = "~/scripts/jquery-1.4.1.min.js",
>>>>
>>>>                DebugPath = "~/scripts/jquery-1.4.1.js",
>>>>
>>>>                CdnPath = "http://ajax.microsoft.com/ajax/jQuery/jquery-1.4.1.min.js",
>>>>
>>>>                CdnDebugPath = "http://ajax.microsoft.com/ajax/jQuery/jquery-1.4.1.js"
>>>>
>>>>            });
>>>>

>>>>
>>>>In which method of Global.asax.cs the above code should go? TIA
>>>
>>>Answer: this code goes to Application_Start method.
>>
>>This was a good answer on my PC (running the application from VS 2012). But when I deploy this project on customer site I get the same dreaded message:
>>

>>WebForms UnobtrusiveValidationMode requires a ScriptResourceMapping for 'jquery'. Please add a  ScriptResourceMapping named 
>>jquery(case-sensitive). 
>>

>>So apparently my Global.asax.cs is not the right place. Where else do I specify "ScriptManager.ScriptResourceMapping"?
>
>Can you add some logging to verify that the Application_Start method is being hit. On some websites I upgraded from .NET 2 to .NET 4 I've had to delete the precompiledweb.config file and the previous bin folder for the new global.asax file to be recognized. There are also several other suggestions at http://stackoverflow.com/questions/1632636/global-asax-not-loading-for-precompiled-asp-net-website such as changing Application_Start to Application_OnStart.

I was thinking exactly the same (earlier) that I need to add some way to know that the Application_Start method fires. I will add a code to create a token file somewhere. And in my case this is also upgrade from .NET 2 to .NET 4 so maybe I am having similar problem. I will check suggestions in the stackoverflow post you referenced.
Thank you very much!