In ASP.NET Web forms there is BundleConfig.cs. But there is another place where bundle are made. The body section of the site.master has ScriptManager where the bundles are set. So actually it is done in two places. So removing/commenting the code in BundleConfig.cs does not completely remove them.
>You can use them or not. In MVC, global.asax calls RegisterBundles, where those bundles are defined. In RegisterBundles, you define which files are bundled. If you take out the call to RegisterBundles, they won't be used. I'm pretty sure it's the same in ASP.Net
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